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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP424872DPRYeast1mgQuote
EP424872DPRE. coli1mgQuote
BP424872DPRBaculovirus200ugQuote
MP424872DPRMammalian Cell200ugQuote

Protein Information

SpeciesAzorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571)
UniProt IDA8IG00
Gene NamemiaB; Locus:AZC_0008
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-464
Expression Tag6xHis
Purity>90%
AA SequenceMQEPRKLYVKSFGCQMNVYDSQRMADALAKEGYVETQDPADADLVILNTCHIREKAAEKV YSELGRLRKAKQDAGSDTTIAVAGCVAQAEGAEIMKRAPVVDLVVGPQSYHRLPEMLARV RDGKRVVDTEFPAEDKFDHLPAPSAAATKKRGPTAFVTVQEGCDKFCTFCVVPYTRGAEV SRSVSKIVGEARALVDQGVREITLIGQNVNAFHGEGPDGRTWGLGRLLEELAGINGLARL RYTTSHPRDMDDGLIAAHRDLPQLMPYLHLPVQSGSDRILAAMNRKHGREIYFEIIDKLR AARPDIALSSDFIVGFPGETEADFEDTLDLARRVGFASAYSFKYSIRPGTPAAEHDHQLS EEVKSERLARLHQVLEASKTAFDRACMGRRFDILLEKPGRLPGQLIGRSPYLQSVVVTAP GAGIGDFVTVDITDVGPNSLAGQVVDAVEDRGRTSDRPLVAMEA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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