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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP390924PYUYeast1mgQuote
EP390924PYUE. coli1mgQuote
BP390924PYUBaculovirus200ugQuote
MP390924PYUMammalian Cell200ugQuote

Protein Information

SpeciesPolynucleobacter sp. (strain QLW-P1DMWA-1)
UniProt IDA4T007
Gene NamemiaB; Locus:Pnuc_1859
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-448
Expression Tag6xHis
Purity>90%
AA SequenceMKKLYIKTFGCQMNEYDSGKMADLLHADEGMVMTDTPEDADVVLLNTCSIREKAEDKVFS DLGRLRELKKTKPHMLIGVGGCVASQEGRQIVSRAPYVDVVFGPQTLHRLSDLIAQRRKT GLSQVDISFPEIEKFDHLPASRQTRGSAYVSIMEGCSKYCSYCVVPYTRGEEVSRPFDDV LTEVAGLASKGVKEIVLLGQNVNAYLGKMGDAEEIADFALLIEYIAEIPGVERIRFTTSH PKEFTQRLIDVYAKVPKLVSHLHLPVQHGSDSILSAMKRGYTALEYKSIIRKMRAVRPDL TLSSDFIVGFPGETDADFEKLLKMVQELDFDNSFCFIFSPRPGTPAANLSDDTPYEVKLK RLQTLLALIEGQSNQISQKMLGKTERVLIEGLAKDGVNLQGRAENNRVIHFSPPDQNIDG LIGEMVDIRITEVLNYTLRGELVETHVI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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