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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP398363VEYYeast1mgQuote
EP398363VEYE. coli1mgQuote
BP398363VEYBaculovirus200ugQuote
MP398363VEYMammalian Cell200ugQuote

Protein Information

SpeciesVibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395)
UniProt IDA5F2X6
Gene NamemiaB; Locus:VC0395_A0484, VC395_0977
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMSKKLLIKTWGCQMNEYDSSKMADLLNAANGYELTEIPEEADVLLLNTCSIREKAQEKVF HQLGRWKTLKDKKPGVVIGVGGCVATQEGDSIRDRAPYVDVIFGPQTLHRLPEMIKQSQT SDAPVMDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCSKYCTYCVVPYTRGEEVSRPMDD VLFEIAQLAEQGVREVNLLGQNVNAYRGATHDGGICSFAELLRLVATIDGIDRIRFTTSH PLEFTDDIIAVYEDTPELVSFLHLPVQSGSDRILTMMKRPHTAIEYKSIIRKLRKARPDI QISSDFIVGFPGETDKDFQDTMKLIRDVDFDMSFSFIFSPRPGTPAADYPCDLSEEVKKE RLYELQQQINSQAMRYSRLMLGTEQRILVEGPSKKDLMELRGRTENNRVVNFEGSPDLIG QFVDVKIVDVFPNSLRGELLRTEQEMNLRIATSPSEMKAKTRREDELGVATFTP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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