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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP399035DHZYeast1mgQuote
EP399035DHZE. coli1mgQuote
BP399035DHZBaculovirus200ugQuote
MP399035DHZMammalian Cell200ugQuote

Protein Information

SpeciesDichelobacter nodosus (strain VCS1703A)
UniProt IDA5EXA7
Gene NamemiaB; Locus:DNO_1245
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-456
Expression Tag6xHis
Purity>90%
AA SequenceMQTVLKHVYIETYGCQMNEYDSSKMLAVLKNSHGITPVATPEEADVLLLNTCSVREKAQE KVFSQLGRWKSLKERKPHLIIGVGGCVASQEGEMIRRRAPEVDVVFGPQTLHRLPNLIEE AQRSRGGVVDVSFPEIEKFDHLPEPRAEGPTAYVSVMEGCSKYCTYCVVPYTRGAEISRP FDDVLAECATLAAQGVREINLLGQNVNAYRGAMHDGTIADLALLIEYVAAIPNIGRIRFT TSHPSEFSDALIETYRRVPKLVSHLHLPVQSGSNRILALMKRDYKVAEYQEKLAKIRAIR PDISFSSDFIVGFPGEEEEDFQATMDLIEAVFFDTSYSFIYSQRPGTPASTMPDRVPLTV KKERLARLQARILEMAASISEAMVGTEQWVLVDRLSRKSEREVSGRTENNRVVNFSAPAS LIGRFAKVQITAAYKNSLRGRLIEAELLPDPVVYTR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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