Request QuoteCatalog Number: xP390160GEHSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP390160GEHYeast1mgQuote
EP390160GEHE. coli1mgQuote
BP390160GEHBaculovirus200ugQuote
MP390160GEHMammalian Cell200ugQuote

Protein Information

SpeciesGeobacillus thermodenitrificans (strain NG80-2)
UniProt IDA4IMH7
Gene NamemiaB; Locus:GTNG_1157
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-523
Expression Tag6xHis
Purity>90%
AA SequenceMNEKQRLEQTGQIQTASHPADRKSDLERLKAKTTKDYEKYFTSVFLPPNLKEAKKRGKEE VQYVKDFTIPDEFRGMGRGRKFYIRTYGCQMNEHDTEVMAGIFMALGYEPTDRPEDANVI LLNTCAIRENAENKVFGELGYLKPLKTTNPDLLLGVCGCMSQEEAVVNKILKQYQYVDLI FGTHNIHRLPYILHEAYMSKEMVVEVWSKEGDVVENLPKVRKGNIKAWVNIMYGCDKFCT YCIVPYTRGKERSRRPEDIIQEVRQLAAQGYKEITLLGQNVNAYGKDFNDIQYGLGDLMD ELRKIDIARIRFTTSHPRDFDDRLIEVLAKRGNLVEHIHLPVQSGSTEILKMMGRKYTRE EYLELVRKIKAAIPDVALTTDIIVGFPNETDEQFEETLSLYREVEFDSAYTFIYSPREGT PAAKMNDNVPMEVKKERLQRLNALVQEIAAKKMKQYEGQVVEVLVEGESKTNPDVLAGYT RKNKLVHFVGPKSLIGQLVNVRITQAKTWTLTGELANEAIEVN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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