Request QuoteCatalog Number: xP385919MNXSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP385919MNXYeast1mgQuote
EP385919MNXE. coli1mgQuote
BP385919MNXBaculovirus200ugQuote
MP385919MNXMammalian Cell200ugQuote

Protein Information

SpeciesMethylibium petroleiphilum (strain PM1)
UniProt IDA2SD55
Gene NamemiaB; Locus:Mpe_A0532
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-457
Expression Tag6xHis
Purity>90%
AA SequenceMSAAGTGKKVFIKTFGCQMNEYDSDKMADVLRAAEGYEPTTDVEQADLILFNTCSVREKA QEKVFSDLGRVKHLKARGVKIGVGGCVASQEGAALIERAPYVDVVFGPQTLHRLPEMLAR RDAQQRPQVDISFPEIEKFDHLPPARVDGATAFVSIMEGCSKYCSYCVVPYTRGEEVSRP FDDVLTEVASLADQGVKEVTLLGQNVNGYRGRMGDTAEIADFALLLEYVAEIPGIARIRY TTSHPNEFSQRLIDVYARVPQLVNHLHLPVQHGSDRILSAMKRGYTSLEYKSSIRKLRAI RPDISLSTDFIVGFPGETDDDHARTMKLIEDVGFDHSFSFIYSARPGTPAAALHDDAPRE LKLARLQQVQEAIEANGRRIGQSRVGTVQRILVEGPSRRPELNAGHELMGRTECNRIVNF AGPARLTGQLIDVTITEAYPHSLRGEVLLRERSPQPA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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