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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP368652MNGYeast1mgQuote
EP368652MNGE. coli1mgQuote
BP368652MNGBaculovirus200ugQuote
MP368652MNGMammalian Cell200ugQuote

Protein Information

SpeciesMagnetococcus sp. (strain MC-1)
UniProt IDA0L7K3
Gene NamemiaB; Locus:Mmc1_1435
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-437
Expression Tag6xHis
Purity>90%
AA SequenceMKHLYIKTFGCQMNSYDSTRMADLLGESHAFQSTDDPEKADLIILNTCHIREKAEDKLFS ELGRLRPLAERGVILAVGGCVGQAEGRTIFSRAPYVRMVFGPQNYHKLPQMIQRALDGET RVIAEDIPSVDKFDNLPQVRAQGVVGQVTVQEGCDKFCAFCVVPYTRGREWSRPVAAILA ETEALAQQGVREVLLLGQNVNAYAGVDEEGVSYDLALLIRRVALIEGIERIRFVTSHPVD MNEDLVEVFGEIEQLAPYLHLPIQSGSDAILAAMQRGHTVEEYCTWVEKVRAVCPDVALA SDFIVGFPGETEQDFQATLDLISRLGFDHAYSFKYSSRPGTPAADMPEQVDEAEKSRRLE RLQQLLNTQQLQRNKARVGRRESVLVEGVSKKRDGELSGRSGTLRTVNFAGPVALIGQFV DVEIVEGLPNSLRGRLV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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