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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP377104STKYeast1mgQuote
EP377104STKE. coli1mgQuote
BP377104STKBaculovirus200ugQuote
MP377104STKMammalian Cell200ugQuote

Protein Information

SpeciesShewanella amazonensis (strain ATCC BAA-1098 / SB2B)
UniProt IDA1S8S1
Gene NamemiaB; Locus:Sama_2575
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMSKKLHIKTWGCQMNEYDSSKMADLLGEYQGYTLTEEAEEADILLLNTCSIREKAQEKVF HQLGRWKTLKDKNPDLIIGVGGCVASQEGKAIKDRAQCVDIIFGPQTLHRLPEMIDQVRS GEKAVIDVSFPEIEKFDRLPEPRAEGPTAFVSIMEGCSKYCSFCVVPYTRGEEVSRPMDD IILEIAQLAEQGVREVNLLGQNVNAYRGAKHDGEICTFAELLRHVAAIDGIDRLRFTTSH PIEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTAMKRGHMAIEYKSIIRRLRKARPDI QISSDFIVGFPGETAQDFQDTMKLIEDVNFDMSFSFIYSARPGTPAADLPDDVDMEEKKQ RLADLQELINQQAMRYSRQMMGTVQRILVEGPSVKNPMELRGRTETNRVVNFEGLHKHIG TFVDVEIVDVFPNSLRGKFIRGEDEMDLRKSLRPEDILAKHQKADDLGVTHFKP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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