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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP400841SVCYeast1mgQuote
EP400841SVCE. coli1mgQuote
BP400841SVCBaculovirus200ugQuote
MP400841SVCMammalian Cell200ugQuote

Protein Information

SpeciesSynechococcus sp. (strain WH7803)
UniProt IDA5GML6
Gene NamemiaB; Locus:SynWH7803_1755
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-454
Expression Tag6xHis
Purity>90%
AA SequenceMLPSRERGSYWITTFGCQMNKADSERMAGILESMGYREASAELDADLVLYNTCTIRDNAE QKVYSYLGRQAQRKRTNPNLTLVVAGCVAQQEGESLLRRVPELDLVMGPQHANRLETLLQ QVDSGQQVVATEEHHILEDITTARRDSSICGWVNVIYGCNERCTYCVVPSVRGKEQSRLP DAIKLEMEGLAAQGFKEITLLGQNIDAYGRDLPGITPEGRRQHTLTDLLHHVHDVEGLER IRFATSHPRYFTDRLIDACADLPKLCEHFHIPFQSGDNDVLRAMARGYTVERYRRIIDRI RERMPDASLSADVIVAFPGETDAQYRRTLALIEEIGFDQVNTAAYSPRPNTPAADWDNQL PEDIKVERLREINALVERCARERNGRYAGRVEEVLAEGINPKDPSQLMGRTRTNRLTFFS ATGADDRRYQAGDLVNVHIDAVRSFSLSGTPLNC
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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