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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP400079GEIYeast1mgQuote
EP400079GEIE. coli1mgQuote
BP400079GEIBaculovirus200ugQuote
MP400079GEIMammalian Cell200ugQuote

Protein Information

SpeciesGeobacter uraniireducens (strain Rf4) (Geobacter uraniumreducens)
UniProt IDA5G670
Gene NamemiaB; Locus:Gura_3127
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-440
Expression Tag6xHis
Purity>90%
AA SequenceMTREKLLYLETFGCQMNVSDSEKIAALLKGIGYFPTQDSSQADLVILNTCSVRAKAEEKV YNHLVQYKGLKRKKPGIILGVGGCVAQQEGERLLANVPHLDIVFGTHNLHLLPELVRAAE KGERLAEVGFIDNETRLDLFPVDERTDGVSRFVTVMQGCENFCSYCIVPYVRGREISRRS ADILGEVRGMAGNGVKEVTLLGQNVNSYGLKSSGEMSFIALLREVSLIPGIERIRFTTSH PKDFSQPLIDCFAEIPKLCRHIHLPAQSGSNAVLAAMNRGYTREEYLGSIARLKAACPSI QITGDIIVGFPGETEEDFQATLSLMEEVRYTDVFSFIYSKRPETKAAGYADEVGQDEKQG RLSRLLDLQRRITLETNKSFVGTVQQVLIEGESRRGGQFYGRTSGNRVVNLAADVSLVGS IVNVMITRGDQNSLQGELCR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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