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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP400768ATZYeast1mgQuote
EP400768ATZE. coli1mgQuote
BP400768ATZBaculovirus200ugQuote
MP400768ATZMammalian Cell200ugQuote

Protein Information

SpeciesAcidiphilium cryptum (strain JF-5)
UniProt IDA5FY82
Gene NamemiaB; Locus:Acry_1353
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-450
Expression Tag6xHis
Purity>90%
AA SequenceMTETASRRTLHITTWGCQMNVYDSGRMADVLRPLGYRQVATQDADMVILNTCHIRERASE KLFSELGRLRALKESRGGAMMIAVAGCVAQAEGAEILARAPHVDLVVGSQAYHRLPELIA EIEAKRRAVIDTDFPAAQKFDLLPEDQASQGPIAFLAIQEGCDKFCTFCVVPYTRGAEAS RPAAAILAEARRLVAGGAREIALLGQNVNAWHGEAPDGATWNLARLLAELADIDGLARLR YTTSHPRDMDAALIAAHRDNPKLMPFLHLPVQSGSDAILARMNRRHGADLFRRIAGELRA ARPDIALSSDFIVGFPGETDADFAATMRLVRETGFALAYSFKYSRRPGTPAADAADQIDE AVKDARLQELQAVLRDQQHAFNRAQVGRSFEVLFTGPGRHPGQSAGRSPYLQPVVVDNAD IPPGTLRTVKIVQSNPNSLMASLTQEQIAA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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