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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP401457DHUYeast1mgQuote
EP401457DHUE. coli1mgQuote
BP401457DHUBaculovirus200ugQuote
MP401457DHUMammalian Cell200ugQuote

Protein Information

SpeciesDehalococcoides sp. (strain BAV1)
UniProt IDA5FPV2
Gene NamemiaB; Locus:DehaBAV1_1195
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-418
Expression Tag6xHis
Purity>90%
AA SequenceMPGYYLWTIGCQMNQAESDRLGRLFELWGYSLADKAEDAELVLVNSCVVREHAENKVVNR LHLLRSLKNENPKLKIALTGCLVGQDISLIKKKFPFVDYIFGPGSMPDWREIPEGFILPL RPPVSANVTIMQGCNNFCTYCVVPYRRGREKSRSIAEIGCEVAELVRRGSREVVLLGQNV DSYGHDLPEKPCLADLLSALHDITGLLRIRFLTSHPKDISQKLIDAMAHLPKVCRSLSLP VQSGDDTILASMRRGYTNQQYRELVERIKTAMPDISLQTDLIVGFPSENEEQFNQSYKLM ADIGYDAIHVAAYSPRPQTVAAHDMADDVPVIEKKRRLKLIEDLQKETVGKANAALMDTF AEVLVEGLQKNKWQGRTLGGKLVFLESDLPLEGCLVKVKIFKTSPWSLQAKLVNIMES
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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