DACT3 antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:250 ~ 1:500 and immunohistochemistry analysis is 1:50. Recommended starting dilution for Western blot is 1:250 and Immunohistochemistry is 1:50.
Mouse IgG1 heavy chain and κ light chain.
Anti-human DACT3 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human DACT3 amino acids 89-202 purified from E. coli.
1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
DACT3 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
12 months at -20oC. 1 month at 4oC.
For periods up to 1 month store at 4oC, for longer periods of time, store at -20oC. Prevent freeze thaw cycles.
Dapper homolog 3, Antagonist of beta-catenin Dapper homolog 3, Arginine-rich region 1 protein, DACT3, RRR1, MGC15476.
Mouse Anti Human Monoclonal.