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Recombinant L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (mshC)

Recombinant L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (mshC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP517130DQWYeast1mgQuote
EP517130DQWE. coli1mgQuote
BP517130DQWBaculovirus200ugQuote
MP517130DQWMammalian Cell200ugQuote

Protein Information

SpeciesCellulomonas flavigena (strain ATCC 482 / DSM 20109 / NCIB 8073 / NRS 134)
UniProt IDD5UFA8
Gene NamemshC; Locus:Cfla_2010
Protein NameL-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase
Region Expressed1-415
Expression Tag6xHis
Purity>90%
AA SequenceMLSWPAPQIPRLPGTGEPVRVLDTATGRLVVAATGPHARLYVCGITPYDATHLGHASTYV AFDVLVRAWLDEGKTVTYASNVTDVDDPLLERATATGVDWRDLAAQQTALYASDMTTLGV VPPDVYRGVVESVPQVVAAVDALLSRDAAYRLPAPDGGDDVYADLSADPGFGSVAGLEHA AMLALSAERGGDPDRPGKRSPLDPLLWRAERPGEPAWDAPGLGRGRPGWHVECAVIASDG LGVPFDVQGGGSDLAFPHHESSASHLRVLTGTPQPAAAHVHTGMVGYRGHKMSKSLGNLV LVSQLVADGVEPMAVRLAVLAHRYRSDWEWTDDVLATAQQRVARWRRALSGNGGPAAQPV LDGVRAAVADDLDTPRALAVVDAWATAALAGEVPFEEGAPGVVARTVDALLGVRM
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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