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Recombinant L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (mshC)

Recombinant L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (mshC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP515162SYEYeast1mgQuote
EP515162SYEE. coli1mgQuote
BP515162SYEBaculovirus200ugQuote
MP515162SYEMammalian Cell200ugQuote

Protein Information

SpeciesSegniliparus rotundus (strain ATCC BAA-972 / CDC 1076 / CIP 108378 / DSM 44985 / JCM 13578)
UniProt IDD6ZFG9
Gene NamemshC; Locus:Srot_1222
Protein NameL-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase
Region Expressed1-420
Expression Tag6xHis
Purity>90%
AA SequenceMRSWLAPSVPKLAGAGPRLRLWDSADRIIRPVTPGRTATMYVCGITPYDATHLGHAATYL AFDLVHRLWLDAGHQVAYAQNITDVDDPLLERADRDHRDWQELAAEQIELFRTDMSALRV LPPHEYVGVTESVGLVVAMVEKLLANQSAYVVAEGEYPDVYHRIAATEDFGYVSGYGREE MAQVFRERGGDPDRPGKADPLDALLWRAARPGEPSWPSPFGPGRPGWHVECSAIALDRIG FGFDVQGGGSDLVFPHHEFSAAHAQAQHGAGAADEHRRFARNYVHAGMIGLDGEKMSKSL GNLVFVSALRERGVDPSAIRLALFAGHYRADRSWDQGLLREAQERLARWRHAASLASGPA AHDVVARMREHLANDLDTPKALAALDSWSLEAAAHGGGDQEAPALVARAADALLGVQLTP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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