Macrophage-Colony Stimulating Factor Mouse Recombinant
Macrophage Colony Stimulating Factor Mouse Recombinant produced in E.coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 156 amino acids and having a total molecular mass of 36.4 KD.
MCSF is purified by proprietary chromatographic techniques.
Accession
Amino acid sequence
MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP.
Biological Activity
The ED50, calculated by the dose-dependant stimulation of the proliferation of murine M-NFS-60 indicator cells was found 0.91ng/ml, corresponding to a specific activity of 1MIU/mg.
Formulation
The protein (1mg/ml) was lyophilized with 5mM Tris, pH 8.0 + 100mM NaCl.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility
It is recommended to reconstitute the lyophilized M-CSF in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Source
Stability
Lyophilized M-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution MCSF should be stored at 4oC between 2-7 days and for future use below
-18oC.
Please prevent freeze-thaw cycles.
Synonyms
CSF-1, Lanimostim, MCSF, M-CSF.
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