The specific activity was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells (Mouse Myeloid Leukemia indicator cell line) and is typically 0.02-0.08ng/ml.
The G-CSF was lyophilized from 1mg/ml in 1xPBS.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 95% as obsereved by SDS-PAGE.
It is recommended to reconstitute the lyophilized G-CSF in sterile water not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Lyophilized G-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution G-CSF should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
CSF-3, MGI-1G, GM-CSF beta, Pluripoietin, Filgrastim, Lenograstim, G-CSF, MGC45931, GCSF.