$549.75Catalog Number: SF0100-25Size: 25 assays

CoVID19, Nucleic acid test

SARS-CoV-2 Nucleic Acid Dual-Detection Kit (One-step qRT PCR Method)

This kit uses one-step qRT-PCR methods to quantitatively detect the conserved regions of the SARS-CoV-2 virus (the virus causes the CoVID19 disease in human). RNA is isolated from subject samples (e.g. nosal and/or mouth swabs) and used in one-step qRT-PCR analysis. The Taqman probes in one-step supermix are designed to bind specifically to the conserved regions of the SARS-CoV-2 virus genome (the N gene and the ORF1ab gene).

To monitor the qualities of sample collection, RNA extraction and PCR operation, the human ribonuclease P (RNP) gene is used in the kit as internal control. This helps avoid false negative to the greatest extent.

The following types of samples have been tested with the kit: throat swab, nasal swab, nasopharyngeal extract, sputum or spit, respiratory tract tissue extract, bronchial perfuate, alveolar lavage, lung tissue extract, stool extract and urine extract.

For more information on the coronavirus disease 2019, please visit the Wikipedia Coronavirus disease 2019 page.

The product is for research use only (RUO), not for in vitro diagnostic (IVD) use.

Kit Components

Component25-reaction Kit50-reaction Kit
N gene/ORF1ab RT-PCR Mix425 ul850 ul
N gene/ORF1ab Probe Mix50 ul100 ul
N gene/ORF1ab Enzyme Mix25 ul50 ul
Negative Control30 ul30 ul
N gene/ORF1ab Positive Control30 ul30 ul

Note: Reagents can only be used with the assigned batch.

Quantity

$549.75

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