Request QuoteCatalog Number: xP526647RLDSize: 0.2-1mg

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Recombinant 4-coumarate--CoA ligase (pcl)

Recombinant 4-coumarate--CoA ligase (pcl) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP526647RLDYeast1mgQuote
EP526647RLDE. coli1mgQuote
BP526647RLDBaculovirus200ugQuote
MP526647RLDMammalian Cell200ugQuote

Protein Information

SpeciesRhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
UniProt IDO69140
Gene Namepcl; Locus:RCAP_rcc01063
Protein Name4-coumarate--CoA ligase
Region Expressed1-384
Expression Tag6xHis
Purity>90%
AA SequenceMVRRLLISLIRAEARRGRNQILPEAAFTGDPRIDEEGLGFDSLARLDLIGAVRDFFDLSR TGIEDYVYVEPTLQGWIDRIMQHFDLLAARSETAQAVFRTSGSTGTPKPIPHPWPKLMRE AASMARDQGLVPAPGGAVIGLVPAHHLFGCLFTALLPELAGAALRDLTAAPPASALRTAQ PGDLIIATPHLWAHLGAAGAFPPGLRGVSSGAPMPDALWHSLLAAGLEDLTEVYGASETG GIGLRRAPGAAFTLLPFLSRSADDGISDGPAPLPLQDRLRWTGPVRFVIEGRLDQALQVG GVNVRLGHVKSVLEAEPGVEALALRLGGDRLKAFVVCAADAEAGLEARLRARAEAGLDAP ARPQHYRFGRALPLTREGKARDWD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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