Standard ELISA test
Immuno-Dot test with positive/negative sera panels.
SDS-PAGE
Western-Blot.
It has been reported that the immunodominant epitope of COL4A3 is a cryptic epitope that is not easily accessible to the corresponding autoantibodies. It is necessary to treat the protein under nonreducing conditions with a denaturant such as urea to unmask the epitopes (see Hellmark et al. in Autoantibodies, Peter, J.B. and Shoenfeld, Y., eds., Elsevier B.V., 1996, pp 291-298).
0.12-0.5 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
The protein solution (0.6 mg/ml) contains 20mM Hepes, pH 8.0, and 4M Urea
Sterile Filtered clear solution.
Recommendations for storage buffer: Ionic strength between 50 and 100 mM, neutral to slightly alkaline pH and 4 M urea as dissociating agent.
Storage temperature is -70¡ã to -80¡ã C.
Please prevent freeze-thaw cycles.
Collagen alpha-3(IV) chain, Goodpasture antigen, COL4A3, Glomerular Basal Membrane, GBM.
