$148.20Catalog Number: PTN203-10ugSize: 10ug

CLDN4 Human

Claudin-4 Human Recombinant

The CLDN4 Protein Human is produced in E. coli, and its molecular weight is 10.63 kDa. The protein containing 93 amino acid residues of the human CLDN4,10 amino acid N-Terminal HisTag residues and 8 additional amino acid PreScission protease cleavage site. The protein is a fusion protein consisting of two extracellular domains of claudin-4 linked together with the PreScission protease LEVLFQGP cleavage site.

Accession

O14493

Amino acid sequence

MKHHHHHHAS MWRVTAFIGS NIVTSQTIWE GLWMNCVVQS TGQMQCKVYD SLLALPQDLQ AAR LEVLFQG PTAHNIIQDF YNPLVASGQK REM MWRVTAFIGS NIVTSQTIWE GLWMNCVVQS TGQMQCKVYD SLLALPQDLQ AAR LEVLFQG PTAHNIIQDF YNPLVASGQK REM

Applications

Western blotting, ELISA.

Formulation

Filtered (0.4 um) and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 90% as determined by SDS-PAGE.

Solubility

It is recommended to add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10ug/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Source

Escherichia Coli.

Stability

Store lyophilized protein at -20oC. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4oC for a limited period of time; it does not show any change after two weeks at 4oC.

Synonyms

Claudin 4, CPE-R, CPE-receptor, Clostridium perfringens enterotoxin receptor 1, WBSCR8, Williams-Beuren syndrome chromosomal region 8 protein, CPETR1.

Quantity

$148.20

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