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Recombinant Class B acid phosphatase (aphA)

Recombinant Class B acid phosphatase (aphA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP520455HTCYeast1mgQuote
EP520455HTCE. coli1mgQuote
BP520455HTCBaculovirus200ugQuote
MP520455HTCMammalian Cell200ugQuote

Protein Information

SpeciesHaemophilus parainfluenzae (strain T3T1)
UniProt IDE1W3A7
Gene NameaphA; Locus:PARA_07400
Protein NameClass B acid phosphatase
Region Expressed23-235
Expression Tag6xHis
Purity>90%
AA SequenceSNKEPYTEQGTNAREMTEQKPIHWISVEQLKKELEGKAPINVSFDIDDTVLFSSPCFYHG QEKYSPGKNDYLKNQDFWNEVNAGCDQYSIPKQIAVDLINMHQARGDQIYFITGRTAGDK DGVTPVLQKAFNIKDMHPVEFMGGRKLPTKYNKTPGIIEHKVSIHYGDSDDDILAAKEAG IRGIRLMRAANSTYQPMPTLGGYGEEVLINSNY
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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