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Recombinant Cholesterol side-chain cleavage enzyme, mitochondrial (CYP11A1)

Recombinant Cholesterol side-chain cleavage enzyme, mitochondrial (CYP11A1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP006389HOYeast1mgQuote
EP006389HOE. coli1mgQuote
BP006389HOBaculovirus200ugQuote
MP006389HOMammalian Cell200ugQuote

Protein Information

SpeciesEquus caballus (Horse)
UniProt IDO46515
Gene NameCYP11A1
Protein NameCholesterol side-chain cleavage enzyme, mitochondrial
Region Expressed40-520
Expression Tag6xHis
Purity>90%
AA SequenceMSSHSPRPFKEIPSPGDNGWINLYHFWREKGPKKLHYHHFQNFQKYGPIYREKLGNVESV YIVDPEDVALLFKFEGPHPERFLIPPWTAYHQYFQKPVGVLFKSSDAWKKDRLALNPEVM ALESIKNFIPLLDPVSQDFVSLLHRRMEQQGSGKFSGPIIEDLFRFAFESITNVIFGERQ GMLDEIVDPEAQRFIDAVYKMFHTSVPMLSLPPDLFRLFRTKTWRDHVAAWDTVFSKAEQ YTEKFYQDLKQKRHFDSYPGIFYRLLASNKLPFKDIQANVTEMLAGGVDTTSMSLQWHLY EIARNLRVQEMLREEVLAARRQAQGDTSTMVQMVPLLKASIKETLRLHPIAVTLQRYPQN DLVIRDYMIPAKTLVQVSIYTMGQDPTFFSNPRRFDPTRWLDKNKDLTHFRNLGFGWGVR QCLGRRIAELEMTLFLIHILENFRVEIQHLNDVDSTFGLILIPEKPISFTFWPITRAPPQ A
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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