Chimeric Chagas Multiantigen Recombinant
Recombinant Chimeric Chagas Multiantigen (MACH) (Trypanosoma cruzi) produced in E. Coli, is a polypeptide chain of 87 a. a. with epitopes PEP-2, TcD, TcE and SAPA. The protein is fused to a 6-His tag and having an Mw of 9.9kDa.
Applications
Suitable for use in ELISA and Lateral flow. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Formulation
Chimeric Chagas Multiantigen was lyophilized from 20mM Tris pH-8.5, 100mM Sodium chloride, 20% trehalose and 0.1% Sodium azide as preservative.
Purification Method
Purified by proprietary chromatographic technique.
Purity
Protein is >90% pure as determined by metal affinity chromatography.
Solubility
It is recommended to reconstitute the lyophilized Chimeric Chagas Multiantigen in sterile 18M?-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Storage
Store lyophilized Chagas Multiantigen at 2-8°C. After reconstitution, store at -20°C. Prevent freeze/thaw cycles.
