Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.
0.15-0.4 ug/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.
CENPA is supplied in 20mM HEPES buffer pH-8, 100mM sodium chloride, and 6M Urea.
1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).
Sterile Filtered liquid formulation.
Greater than 90% as determined by SDS-PAGE.
Store at 4oC if entire vial will be used within 2-4 weeks.
Store, frozen at -20oC for longer periods of time.
Avoid multiple freeze-thaw cycles.
Histone H3-like centromeric protein A, Centromere protein A, CENP-A, Centromere autoantigen A, CENPA.
