Up to 20 ug/ml CD14 inhibit binding of FITC-LPS (0.5ug/ml) to 6 x 105 CD14+CHO transfectants (FACS).
CD14 was lyophilized from a concentrated protein solution (1.0 mg/ml) containing phosphate-buffered saline, pH 7.2.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 95.0% as determined by SDS-PAGE.
It is recommended to reconstitute the lyophilized CD14 in sterile 18MΩ-cm H2O not less than 100ug/ml. Further dilutions should be made with phosphate buffered saline (PBS).
Lyophilized CD14 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution CD14 should be stored at 4oC between 2-7 days and for future use below
-18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14.
