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Recombinant 6-carboxyhexanoate--CoA ligase (bioW)

Recombinant 6-carboxyhexanoate--CoA ligase (bioW) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP513958VEOYeast1mgQuote
EP513958VEOE. coli1mgQuote
BP513958VEOBaculovirus200ugQuote
MP513958VEOMammalian Cell200ugQuote

Protein Information

SpeciesVeillonella parvula (strain ATCC 10790 / DSM 2008 / JCM 12972 / Te3) (Veillonella alcalescens)
UniProt IDD1BMT0
Gene NamebioW; Locus:Vpar_1006
Protein Name6-carboxyhexanoate--CoA ligase
Region Expressed1-242
Expression Tag6xHis
Purity>90%
AA SequenceMDELYSVRMRAAQGGPHENGGHHISGAERIVTLNQVGFIAQSLAERALHHSKGTADFINI TVDLIPSETITYIDCLKVKEHTANTVTEAHQLAVKLLQGTDISESAIRNSIFLLKSLVSS MRGAMLVDAISGERLDAGNRGVRVSHMDSFDSDKLGDNEHMREALVLASKVQSAEGIVGE LCWSDDPDYTIGYVACNGVYHRIPNMKEIGSNLGGRVFFVKPNIDLEGVIEYLEKEPVLV QW
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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