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Recombinant 6-carboxyhexanoate--CoA ligase (bioW)

Recombinant 6-carboxyhexanoate--CoA ligase (bioW) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP511386FERYeast1mgQuote
EP511386FERE. coli1mgQuote
BP511386FERBaculovirus200ugQuote
MP511386FERMammalian Cell200ugQuote

Protein Information

SpeciesFibrobacter succinogenes (strain ATCC 19169 / S85)
UniProt IDC9RPK4
Gene NamebioW; Locus:Fisuc_2956, FSU_0221
Protein Name6-carboxyhexanoate--CoA ligase
Region Expressed1-260
Expression Tag6xHis
Purity>90%
AA SequenceMDYYSLKMRASQHVGEGENSHEQHISGAERIVGRDSVEAVCAAMVRRAMNHSKGDPDFIN VKIEKVHESDIQVLKSLPVTRVDVETWQEGLEKAFGLITPLMALRQAQGPCDGAKKFKEK LQDLLRETFPMRGAMLYDIATGNRLEPDKDRGVRATYMDALHSSEVDGCKNHFNEAIVLA TKVANAPGMVAEFCVSDDPNYVTGYVASKELGYVRIMKMKEMGDENGGRIFLFDSRKASA EECIEYLQKKKVLVDVVGRT
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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