Request QuoteCatalog Number: xP521701BVWSize: 0.2-1mg

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Recombinant 3-carboxy-cis,cis-muconate cycloisomerase (pcaB)

Recombinant 3-carboxy-cis,cis-muconate cycloisomerase (pcaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP521701BVWYeast1mgQuote
EP521701BVWE. coli1mgQuote
BP521701BVWBaculovirus200ugQuote
MP521701BVWMammalian Cell200ugQuote

Protein Information

SpeciesBradyrhizobium japonicum (strain USDA 110)
UniProt IDO31385
Gene NamepcaB; Locus:blr5667
Protein Name3-carboxy-cis,cis-muconate cycloisomerase
Region Expressed1-451
Expression Tag6xHis
Purity>90%
AA SequenceMSTALSPLLAPMLSSAAMRAACDDRSTLQNMLDFEAALARAEAATGVIPAAAVGPIETAC KAATFDIAALAEAATRSGNLAIPLVKALTANVAKADAEAARYVHWGATSQDVIDTATMLT LRAAIDALDADLSRAIKGFAALARNHRNTAMVARTWLQHALPMPFGLKAAEYAASLARAR CRLRRLSRDGLALQFGGAAGTLAALGDKGLVVAERLAQELNLPLPEAPWHTHRDRIAEAA SALAILAGSCGKIARDVSLMMQTDVAEAFEPAGEGRGGSSTMPHKRNPVAAASALGCATM APQLAATIFAAQVQDHERSAGPWHAEWPTLPQLMLVTSGALAAIVDIAEGLDVDAARMRS NLDATHGLIMAEAVTFALAERIGKSDAHHLIEAASKRAVAEKKHLREVLAADSQVTAHLS PEKIAALFEPMAYQGASQAMIDRLLDSLERD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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