$199.50Catalog Number: PTN363-2ugSize: 2ug

CAPN1

Human Calpain-1 Catalytic Subunit

CAPN1 consists of an 80-kDa large subunit and a 30 kDa small subunit.
CAPN1 was purified by sequential chromatography through DEAE-Sepharose, A1.5m Bio-Gel, and Phenyl-Sepharose CL-4B columns.

Accession Number

P07384

Applications

This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.
For adsorption we suggest the following procedure: A- Dilute 1ul of the antiserum against u-calpain in 1ml of the usual buffer for immunohistochemistry (final dilution 1:1000). B- Add 1ug of protein to 1ml of the diluted antibody solution and mix well. C- Incubate for at least 6 hours in the cold. D- Apply to tissue-sections and incubate for 3 days. E - Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB). As a result, the immunostaining should be strongly reduced or even completely prevented.

Formulation

50mM imidazole-HCl, 100mM NaCl, 5mM EGTA, 1mM DTT and 10% sucrose.

Physical Appearance

Sterile Filtered colorless solution.

Purity

Greater than 90% as determined by SDS-PAGE.

Source

Human Erythrocytes.

Stability

CAPN1 although stable at 10oC for 1 week, should be stored desiccated below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

Calpain-1 catalytic subunit, EC 3.4.22.52, Calpain-1 large subunit, Calcium-activated neutral proteinase 1, Calpain mu-type, muCANP, Micromolar-calpain, Cell proliferation-inducing gene 30 protein, CANP 1, CAPN1, CANPL1, PIG30, CANP, muCL, CANP1.

Quantity

$199.50

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