Immunohistochemistry, flow cytometry.
5-iodouridine covalently coupled to KLH.
This antibody was produced in BALB/c mice. This antibody can be used to detect proliferating cells in living tissues after incubation with BrdU which will be incorporated into newly synthesized DNA of replicating cells (during the S phase of the cell cycle), in place of thymidine during DNA replication. Antibody binding requires prior denaturation of the DNA, by exposing the cells to acid or heat.
1mg/ml in PBS (after reconstitution).
Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
Antibody is shipped lyophilized at ambient temperature.
In lyophilized form, for long periods, store at 4oC in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20oC.
Mouse Antibody Monoclonal.