Borrelia Afzelii Outer Surface Protein A Recombinant
Recombinant Borrelia Afzelii Outer Surface Protein A produced in E. coli is a non-glycosylated, polypeptide chain having a calculated molecular mass of 29,074 Dalton. Borrelia Afzelii OspA is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.
Applications
Western blot with Lyme positive plasma.
Formulation
Borrelia Afzelii OspA is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol.
Immunological Functions
1.Binds IgG- and IgM-type human antibodies.2. Immunodot test with Lyme disease positive/negative plasma; suitable for LTT (lymphocyte transformation test).
Physical Appearance
Sterile Filtered clear solution.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Source
Storage
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
