Bone Morphogenetic Protein-4 Human Recombinant
Bone Morphogenetic Protein-4 Human Recombinant produced in E.Coli is a monomeric, non-glycosylated, Polypeptide chain containing 116 amino acids and having a molecular mass of 13009 Dalton.
The BMP-4 is purified by proprietary chromatographic techniques.
Accession
Amino acid sequence
SPKHHSQRAR KKNKNCRRHS LYVDFSDVGW NDWIVAPPGY QAFYCHGDCP FPLADHLNST NHAIVQTLVN SVNSSIPKAC CVPTELSAIS MLYLDEYDKV VLKNYQEMVV EGCGCR.
Applications
1. Molecular standard (Western, ELISA) in studying secreted BMP-4.
2. Preparing antibodies for BMP-4 monomer.
3. Molecule standard in detecting secreted BMP-4 in reduced SDS-PAGE.
Formulation
BMP-4 was lyophilized from a 0.2um filtered concentrated (1mg/ml) solution in 30% Acetonitrile and 0.1% TFA.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility
It is recommended to reconstitute the lyophilized Bone Morphogenetic Protein-4 in sterile 100mM AcOH (acetic Acid) not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Source
Stability
Lyophilized Bone Morphogenetic Protein-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution BMP4 should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Synonyms
BMP4, ZYME, BMP2B, BMP2B1.
