$148.20Catalog Number: PYT276-10ugSize: 10ug

BMP 7 Human, CHO

Bone Morphogenetic Protein-7 Human Recombinant, CHO

The DNA sequence encoding the human BMP-2 signal peptide and propeptide (1~282 amino acid) fused to the N terminus of human rhBMP-7 mature chain (293~431 amino acid) was expressed in a Chinese hamster ovary cell line. The mature recombinant BMP-7 generated by the proteolytic removal of the signal peptide and propetide contains 139 amino acid residues. The glycosylation of BMP-7 increases the molecular mass and the glycosylated proteins migrate as 25 ~ 40 kDa in SDS-PAGE under non-reducing conditions.
BMP-7 is purified by proprietary chromatographic techniques.

Accession

P18075

Biological Activity

Measured in alkaline phosphatase activity assay using MC3T3-E1 cells. The ED50 for this effect is <100ng/ml corresponding to a Specific Activity of 10,000IU/mg.

Formulation

BMP-7 was lyophilized from a concentrated (1mg/ml) sterile solution containing 1% sucrose, 1.2% mannitol, 20mM glycine, and 0.05% tween 20 pH-4.

Purity

Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized Bone Morphogenetic Protein-7 in 1ml sterile/ endotoxin free water.

Source

Chinese Hamster Ovarian Cells (CHO cell).

Stability

Lyophilized BMP-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution BMP 7 Human should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

Osteogenic Protein 1, BMP-7.

Quantity

$148.20

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