Bone Morphogenetic protein-2, Mouse Anti-Human
Monoclonal Antibody
Monoclonal antibodies provide the best target specificity and affinity. It is also advantagous for long term projects as there is little lot to lot variation, where polycolonal antibodies will vary significantly with different preparations due to immunogen preparation and animal responses.
Applications
Applied in detections of antigen or antibody: ELISA or Western blot. For indirect ELISA, dilute antibody solution by 1: 250.
Background
Bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor beta(TGF-f3) superfamily.
It can induce bone formation and regeneration during early embryonic development.
BMP-2 is synthesized as large precursor molecule firstly and the mature protein is secreted by proteolytic cleavage. Natural mature BMP-2 monomer has 114 amino acid residues and has no biological function unless it turns into dimmer. It's assumed that mature BMP-2 forms a cysteine knot with seven conserved cysteine residues. The active form can consist of a dimmer of two identical proteins or a heterodimer of two related bone morphogenetic proteins.
Formulation
Samples are lyophilized in sterile PBS buffers.
Host
Purity
Greater than 90%, analysed by reducing SDS-PAGE.
Source
The BMP-2 antibody was prepared by affinity chromatography from ascites of BALB/c mouseinoculated by a hybridoma cell that secreted anti- BMP-2 monoclonal antibodies.
Storage
Stable at -20oC for two years. For long-term storage, keep aliquot samples at -70oC. Once reconstituted, samples are stable at 4oC for several weeks.
Synonyms
Type
Mouse Anti Human Monoclonal.
