Request QuoteCatalog Number: xP522139EYTSize: 0.2-1mg

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Recombinant Biotin biosynthesis bifunctional protein BioWF (HMPREF0675_4919)

Recombinant Biotin biosynthesis bifunctional protein BioWF (HMPREF0675_4919) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP522139EYTYeast1mgQuote
EP522139EYTE. coli1mgQuote
BP522139EYTBaculovirus200ugQuote
MP522139EYTMammalian Cell200ugQuote

Protein Information

SpeciesPropionibacterium acnes (strain SK137)
UniProt IDD4H9Y2
Gene NameLocus:HMPREF0675_4919
Protein NameBiotin biosynthesis bifunctional protein BioWF Including the following 2 domains: 6-carboxyhexanoate--CoA ligase
Region Expressed1-668
Expression Tag6xHis
Purity>90%
AA SequenceMVFNCYPVANAEESPMSSQNPTTVAWWSVRMRATGTLDDEPYHVSGAESLVAPGMIEQTV AGLTQRALAPGHTVKARQVRVSLDQLDVEPTIIPALPTELKECPDPVAARQYFVDVLSRF VPHPAEALRVLTEGPTMRGAAMVEAGTDRRLEADPLRGVRVTKFGDLTESAPGASLAHKK HHHEAVLLASKVAAAPGVLAEFCISDDPHYTRGYVCVDGVYTTVTNVKADGDPNGGRVIL VDTARADPTTITTWLENHPVLIGPATASSQKATSWHGHLCGRLNAWRAAGLERRPRTFCS AQDPDAVTTDGPALLFSSSDYLGLSTEPKVQQAMNNTVRRLGSSSGGSRLTTGTSVAHHQ AEHEIAAWLGYPQAVFMASGYQANIATIQLLADPHVTVISDAENHASLIDGCRLARARTV VVPHADLDVIDTALDCVTTDRALVLTEGVYSMGGDVAPVGELVEIAHRHGALVVVDDAHG IGTVGPTGRGATEELPASQRPDVLLGTASKALGVEGGFACLDETLATLMRNCARGYVFSS APSPVVAAGVAAAVEYLRTDTRRVCSLQANVAQARLLLAEADLIPPSAAHDRGPIIRIPV GPESRAVAAQEELARRGLMVGAIRYPAVARGDAILRICLTARHTDEHIRILVTSLREVLD GALSDAPR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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