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Recombinant Biotin biosynthesis bifunctional protein BioAB (bioB)

Recombinant Biotin biosynthesis bifunctional protein BioAB (bioB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP515196BRXYeast1mgQuote
EP515196BRXE. coli1mgQuote
BP515196BRXBaculovirus200ugQuote
MP515196BRXMammalian Cell200ugQuote

Protein Information

SpeciesBacteroides fragilis (strain 638R)
UniProt IDE1WTS4
Gene NamebioB; Locus:BF638R_1618
Protein NameBiotin biosynthesis bifunctional protein BioAB Including the following 2 domains: Biotin synthase BioB
Region Expressed1-748
Expression Tag6xHis
Purity>90%
AA SequenceMTIEEIKNQVLQGTAISREQAEWLALYPRKEELYDAAHDITTACASQEFDMCSIINARSG RCPENCKWCAQSSHYKTKADVYDLVSAEECLRQAKYNEAQGVNRFSLVTSGRKPSPKNMK ELCVAARRMRRHSSIRLCASLGLLDEEELQALYDAGVTRYHCNLETAPSHFDSLCTTHTQ EQKLKTLHAARRVGMDLCCGGIIGMGETVEQRIEFAFTLRDLNIQSIPINLLQPIPGTPL EHQSPLSEEEILTTVALFRFINPAAYLRFAGGRSQLTPEAVRKSLYIGINSAIVGDLLTT LGSKVSDDKEMILSEGYHFADSQFDREHLWHPYTSTSNPLPVYKVKRADGATITLESGQT LIEGMSSWWCAVHGYNHPILNQAVQDQLSRMSHVMFGGLTHDPAIELGKLLLPLVPPSMQ KIFYADSGSVAVEVALKMAVQYWYAAGKPEKNNFVTIRNGYHGDTWNAMSVCDPVTGMHS IFGSALPIRHFLPAPSSRFGDEWNPEDIRPLEHLLEKHTDELAAFILEPIVQGAGGMRFY HPEYLREAARLCHRYGVLLIFDEIATGFGRTGKLFAWEHAGVEPDIMCIGKALTGGYMTL SAVLTTNEVADCISNHAPGAFMHGPTFMGNPLACAVACASVRLLLTSGWQENVKRIEAQL NRELAPARELPQVADVRVLGAIGVIEMKEPVNMAYLQRRFVEEGIWLRPFGKLIYVMPPF IITPEQLTKLTEGMIRIISNGLPGSQTK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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