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Recombinant 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase (MVA157L, ACAM3000_MVA_157)

Recombinant 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase (MVA157L, ACAM3000_MVA_157) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP523873VEEYeast1mgQuote
EP523873VEEE. coli1mgQuote
BP523873VEEBaculovirus200ugQuote
MP523873VEEMammalian Cell200ugQuote

Protein Information

SpeciesVaccinia virus (strain Ankara) (VACV)
UniProt IDO57245
Gene NameLocus:MVA157L, ACAM3000_MVA_157
Protein Name3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase
Region Expressed1-346
Expression Tag6xHis
Purity>90%
AA SequenceMAVYAVTGGAGFLGRYIVKLLISADDVQEIRVIDIVEDPQPITSKVKVINYIQCDINDFD KVREALDGVNLIIHTAALVDVFGKYTDNEIMKVNYYGTQTILAACVDLGIKYLIYTSSME AIGPNKHGDPFIGHEHTLYDISPGHVYAKSKRMAEQLVMKANNSVIMNGAKLYTCCLRPT GIYGEGDKLMKVFYEQCKQHGNIMYRTVDDNAVHSRVYVGNAAWMHVLAAKYIQYPGSEI KGNAYFCYDYSPSCSYDMFNLLLMKPLGIEQGSRIPRWMLKMYACKNDMKRILFRKPSLL NNYTLKISNTTFEVRTNNAELDFNYSPIFNVDVAFERTRKWLEESE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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