KKVYNAVSCM TNGGICWLKC SGTFREIGSC GTRQLKCCKK K.
Measured by its antimicrobial activity against E. coli. The ED50 for this effect is typically 4-20ug/ml, corresponding to a specific activity of 50,000-250,000units/mg.
BD-3 protein was lyophilized from a 0.2um filtered concentrated solution in PBS, pH 7.4.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
It is recommended to reconstitute the lyophilized BD-3 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Lyophilized BD-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution BD-3 should be stored at 4oC between 2-7 days and for future use below -18oC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Beta-defensin 3, BD-3, Defensin beta 3, Defb3
