$148.20Catalog Number: PYT063-20ugSize: 20ug

BD 3 Rat

Beta Defensin-3 Rat Recombinant

BD-3 Rat Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 41 amino acids and having a molecular mass of 4.5kDa.
The BD-3 is purified by proprietary chromatographic techniques.

Accession

Q32ZI4

Amino acid sequence

KKVYNAVSCM TNGGICWLKC SGTFREIGSC GTRQLKCCKK K.

Biological Activity

Measured by its antimicrobial activity against E. coli. The ED50 for this effect is typically 4-20ug/ml, corresponding to a specific activity of 50,000-250,000units/mg.

Formulation

BD-3 protein was lyophilized from a 0.2um filtered concentrated solution in PBS, pH 7.4.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized BD-3 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Escherichia Coli.

Stability

Lyophilized BD-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution BD-3 should be stored at 4oC between 2-7 days and for future use below -18oC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

Beta-defensin 3, BD-3, Defensin beta 3, Defb3

Quantity

$148.20

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