KKINNPVSCL RKGGRCWNRC IGNTRQIGSC GVPFLKCCKR K.
Measured by its antimicrobial activity against E. coli. The ED50 for this effect is typically 4-20ug/ml, corresponding to a specific activity of 50-250units/mg.
The Mouse BD-3 was lyophilized from a 0.2um filtered concentrated solution in PBS, pH7.4.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
It is recommended to reconstitute the lyophilized Beta Defensin-3 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Lyophilized Beta Defensin-3 Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution BD-3 should be stored at 4oC between 2-7 days and for future use below -18oC.
Please prevent freeze-thaw cycles.
Beta-defensin 3, BD-3, mBD-3, Defensin beta 3, Defb3, Bd3, MGC129397.