$148.20Catalog Number: PTN446-10mgSize: 10mg

Avidin Biotin

Modified Affinity Purified Avidin

Avidin is a basic charged glycoprotein present in the egg white and in some extent in tissues of various animals. Avidin is a homotetrameric protein that contains 4 identical subunits which bind to Biotin with high extent of affinity and specificity consisting of 128 amino acids and has a molecular mass of about 67K Daltons.
Avidin-Biotin is a modified form of affinity purified avidin that combines the high affinity specific activity of the native egg white avidin to biotin molecule together with a very low background of the streptavidin produced by the bacteria Streptomyces avidinii.

Accession Number

P02701

Activity

Binding activity of Avidin-Biotin is 10-15units/mg protein.
One unit of Avidin activity is defined as the amount of protein which will bind 1ug d-biotin.

Advantages

1. Avidin-Biotin does not exhibit unwanted non-specific binding that have been previously reported for the native egg white avidin. 2. The non-specific properties of Avidin-Biotin have been tested in ELISA, Western Blot & Immunohistochemistry techniques and have been found to be superior to unmodified avidin. 3. Avidin-Biotin rarely affected by pH values between the range of pH-2 to pH-12 or by guanidine-HCl concentrations up to 8M at neutral pH.

Applications

Avidin-Biotin may be used in combination with biotin in various techniques such as ELISA, Western blotting, immunohistochemistry etc¡­ Physical AppearanceLyophilized freeze dry Powder.

Formulation

Salt-free lyophilized powder with no preservative added.

Solubility

We highly recommended to prepare a 1 mg/ml solution; Reconstitute using cold deionized pyrogen free water and mix gently until powder is completely dissolved.

Stability

Store protein solution at 2-8oC.

Synonyms

Avidin, AVD, AVID.

Quantity

$148.20

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