Request QuoteCatalog Number: xP509821EKFSize: 0.2-1mg

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Recombinant ATP-dependent RNA helicase ded1 (ded1)

Recombinant ATP-dependent RNA helicase ded1 (ded1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP509821EKFYeast1mgQuote
EP509821EKFE. coli1mgQuote
BP509821EKFBaculovirus200ugQuote
MP509821EKFMammalian Cell200ugQuote

Protein Information

SpeciesEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
UniProt IDC8V8H4
Gene Nameded1; ORFs:AN10557
Protein NameATP-dependent RNA helicase ded1
Region Expressed1-668
Expression Tag6xHis
Purity>90%
AA SequenceMADGLNMGSLTLNDSQHAPAPGGPPSGGRAAYIPPHLRQRQVNANGDGASAPPPGPTGGS WGGPRGPRGGNWANANAPDFNPRGPNGNTNTNSGWTATEAGRPLFNPNAYGNPGHGGGYA GASARGSGDGQWRDGKHIPGPPNPRLERELFGVPNDPTKQNTGINFANYDDIPVEASGHD VPEPVNTFTNPPLDDHLISNIALARYQTPTPVQKYSIPIVMNGRDLMACAQTGSGKTGGF LFPILSQAYQNGPAAPPPSAAGQFGRQRKAYPTSLILAPTRELVSQIFDEARKFAYRSWV RPCVVYGGADIGSQLRQIERGCDLLVATPGRLVDLIERGRISLVNIKYLILDEADRMLDM GFEPQIRRIVEGEDMPNVNDRQTLMFSATFPRDIQMLARDFLKDYVFLSVGRVGSTSENI TQKVEYVEDHDKRSVLLDILHTHGTTGLTLIFVETKRMADALSEFLINQRFPATAIHGDR TQRERERALEMFRSGRYPILVATAVAARGLDIPNVTHVINYDLPTDIDDYVHRIGRTGRA GNTGIATAFFNRGNRGVVRDLIDLLKEAHQEVPSFLESIAREGSGYGGRGGRGGRGRGAN ATRDMRRMGGGMGGPPSYGGGSGFGAPASNYGGGYGGAPSYGGAGYGGGYGGGGYGNPSG STGPSSWW
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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