4.6 TIU/mg. (Trypsin Inhibitory Unit)
Aprotinin inhibits the activity of several proteolytic enzymes such as chymotrypsin, kallikrein, plasmin and trypsin. Aprotinin is present in blood and in most tissues, with a high concentration in lung. Aprotinin inhibits pro-inflammatory cytokine release and maintains glycoprotein homeostasis. In platelets, aprotinin reduces glycoprotein loss (e.g., GpIb, GpIIb/IIIa), while in granulocytes it prevents the expression of pro-inflammatory adhesive glycoproteins (e.g., CD11b).
The protein was lyophilized without any additives.
Sterile Filtered lyophilized powder.
Greater than 90.0% as determind by Gel Electrophoresis.
It is recommended to reconstitute the Aprotinin in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Aprotinin although stable at room temperature for 3 weeks, should be stored at 4oC.
Pancreatic trypsin inhibitor, Basic protease inhibitor, BPI, BPTI, Aprotinin, AP.
One Trypsin Inhibitory Unit (TIU) is equal to the amount of inhibitor with the ability to inhibit two trypsin units by 50% where one trypsin unit will hydrolyze 1.0 mmole of N-alpha-benzoyl-DL-Arginine-p-Nitroanilide (BAPNA) per minute at pH 7.8 at 25oC.
