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Recombinant APOBEC1 complementation factor (A1CF)

Recombinant APOBEC1 complementation factor (A1CF) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001002PYXYeast1mgQuote
EP001002PYXE. coli1mgQuote
BP001002PYXBaculovirus200ugQuote
MP001002PYXMammalian Cell200ugQuote

Protein Information

SpeciesPongo abelii (Sumatran orangutan)
UniProt IDQ5R9H4
Gene NameA1CF; aka: ACF
Protein NameAPOBEC1 complementation factor
Region Expressed1-587
Expression Tag6xHis
Purity>90%
AA SequenceMESNHKSGDGLSGTQKEAALRALIQRTGYSLVQENGQRKYGGPPPGWDAAPPERGCEIFI GKLPRDLFEDELIPLCEKIGKIYEMRMMMDFNGNNRGYAFVTFSNKLEAKNAIKQLNNYE IRNGRLLGVCASVDNCRLFVGGIPKTKKREEILSEMKKVTEGVVDVIVYPSAADKTKNRG FAFVEYESHRAAAMARRKLLPGRIQLWGHPIAVDWAEPEVEVDEDTMSSVKILYVRNLML STSEEMIEKEFNNIKPGAVERVKKIRDYAFVHFSNREDAVEAMKALNGKVLDGSPIEVTL AKPVDKDSYVRYTRGTGGRGTMLQGEYTYSLGQVYDPTTTYLGAPVFYAPQAYAAIPSLH FPATKGHLSNRAIIRAPSVRGAAGVRGLGGRGYLAYTGLGRGYQVKGDKREDKLYDILPG MELTPMNPVTLKPQGIKLAPQILEEICQKNNWGQPVYQLHSAIGQDQRQLFLYKITIPAL ASQNPAIHPFTPPKLSAYVDEAKTYAAEYTLQTLGIPTDGGDAGTMATAAAVATAFPGYA VPNATAPVSAAQLKQAVTLGQDLAAYTTYEVYPTFAVTARGDGYGAF
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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