RKGFWDYFSQ TSGDKGRVEQ IHQQKMAREP ATLKDSLEQD LNNMNKFLEK LRPLSGSEAP RLPQDPVGMR RQLQEELEEV KARLQPYMAE AHELVGWNLE GLRQQLKPYT MDLMEQVALR VQELQEQLRV VGEDTKAQLL GGVDEAWALL QGLQSRVVHH TGRFKELFHP YAESLVSGIG RHVQELHRSV APHAPASPAR LSRCVQVLSR KLTLKAKALH ARIQQNLDQL REELSRAFAG TGTEEGAGPD PQMLSEEVRQ RLQAFRQDTY LQIAAFTRAI DQETEEVQQQ LAPPPPGHSA FAPEFQQTDS GKVLSKLQAR LDDLWEDITH SLHDQGHSHL GDPAAADYKD DDDK
Cell culture and/or animal studies, ELISA and Western blotting
APOA5 Human was filtered (0.4um) and lyophilized from 0.5 mg/ml supplied in 20mM TRIS and 50mM NaCl, pH 7.5
Greater than 70% as determined by SDS-PAGE.
BCA - to determine content of the protein.
SDS PAGE - to determine purity of the protein.
It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it on cell culture.
Store lyophilized APOA5 Human at -20oC. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted APOA5 Human can be stored at 4oC for a limited period of time; it does not show any change after two weeks at 4oC.
Apolipoprotein A-V, RAP3, Regeneration-associated protein 3, APOA-V, MGC126836, FLJ97995.