Apoliportein-D Human Recombinant
Apoliprotein-D Human Recombinant His Tag fusion protein at C-terminus (7 highlighted a.a.) produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 174 amino acids and having a molecular mass of 19.8 kDa.
The protein a.a.sequence corresponds to the UniProtKB/Swiss-Prot entry P05090.
The Following gene modifications were madeTrp99His, Cys116Ser, Ile118Ser, Leu120Ser amino acids exchanges were introduced at the surface of Apoliprotein-D to enhance the protein's solubility and another three Leu23Pro, Pro133Val, Asn134Ala amino acids exchnages which facilitate its genetic manipulation. The Apoliprotein-D is purified by proprietary chromatographic techniques.
Accession
Amino Acid Sequence
FHLGKCPNPP VQENFDVNKY PGRWYEIEKI PTTFENGRCI QANYSLMENG KIKVLNQELR ADGTVNQIEG EATPVNLTEP AKLEVKFSWF MPSAPYHILA TDYENYALVY SCTSISQSFH VDFAWILARN VALPPETVDS LKNILTSNNI DVKKMTVTDQ VNCPKLSAHHHHHH.
Formulation
Lyophilized from 1mg/ml in 4mM KH2PO4, 16mM Na2HPO4 and 115mM NaCl pH7.5.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95% as determined by SDS-PAGE.
Solubility
Add deionized H2O to a working volume of 0.5mg/ml and let the lyophilized pellet dissolve completely.
Source
Stability
Store lyophilized protein at -20oC. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4oC for a limited period of time; it does not show any change after two weeks at 4oC.
Synonyms
Apolipoprotein D, Apo-D, ApoD.
