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Recombinant 2-aminoethylphosphonate--pyruvate transaminase (phnW)

Recombinant 2-aminoethylphosphonate--pyruvate transaminase (phnW) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP386584BPQYeast1mgQuote
EP386584BPQE. coli1mgQuote
BP386584BPQBaculovirus200ugQuote
MP386584BPQMammalian Cell200ugQuote

Protein Information

SpeciesBurkholderia mallei (strain NCTC 10247)
UniProt IDA3MCV7
Gene NamephnW; Locus:BMA10247_A0895
Protein Name2-aminoethylphosphonate--pyruvate transaminase
Region Expressed1-369
Expression Tag6xHis
Purity>90%
AA SequenceMPERDPILLTPGPLTTSRMTRDAMLRDWGSWDAAFNRLTKSVCADLVRIAGGGDAYVCVP LQGSGTFAVEATLGTLVPRDARVLVPNNGAYCARIAAILRRLGIAHVELPFAEDEPASAH AIDAALARDARLTHVALVHLETSAGLLNPLDDIAAVCRARGRALIVDAMSSFGALPIALA ASGIDALISASGKCLEGVPGMGFAIVRRSALEAAEGRSPSVALDLHDQYAYMQRTSQWRF TPPTHVLAALRAALDQFFDEGGQPARGARYARNCATLVDGMRALGFEPFLDARAQASVIV TFYAPADPAYAFPAFYAAVRDAGYVLYPGKLTTADTFRVGCIGALGADEMRGAVAAIGGA LESLGIAMR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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