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Recombinant 2-aminoethylphosphonate--pyruvate transaminase (phnW)

Recombinant 2-aminoethylphosphonate--pyruvate transaminase (phnW) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP371183BOMYeast1mgQuote
EP371183BOME. coli1mgQuote
BP371183BOMBaculovirus200ugQuote
MP371183BOMMammalian Cell200ugQuote

Protein Information

SpeciesBacillus thuringiensis (strain Al Hakam)
UniProt IDA0RBE9
Gene NamephnW; Locus:BALH_1190
Protein Name2-aminoethylphosphonate--pyruvate transaminase
Region Expressed1-365
Expression Tag6xHis
Purity>90%
AA SequenceMTENHYLLLTPGPLTTTKTVKEVMLYDWCTWDVEYNMMVQKVRAKLVSLATKEEEKYTTV LMQGSGTFSVEAVIGSVIPKNGKLLVCTNGAYGKRIVQMAEMLHIDVVVSQTEEWEPTNI VEVEKILQQDKEITHIAVVHCETTTGIINPIVDVCKLGKQYGKVTLVDAMSSFGGIEIDI AELEIDFLISSANKCIQGVPGFGFVIAQRDELLKCKGQARSLSLDLYDQWETMENQNGKW RFTSPTHVVHAFYQALLELEKEGGVRARYNRYYNNQKLLVNRMGEIGFKPLVNEKYQSPI ITSFIYPEGNFEFQQLYNELKRYGFVIYPGKISKVDTFRIGNIGDVHEEDINRLVDSIAK GVVIG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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