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Recombinant 2-aminoethylphosphonate--pyruvate transaminase (phnW)

Recombinant 2-aminoethylphosphonate--pyruvate transaminase (phnW) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP369025AUHYeast1mgQuote
EP369025AUHE. coli1mgQuote
BP369025AUHBaculovirus200ugQuote
MP369025AUHMammalian Cell200ugQuote

Protein Information

SpeciesAeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240)
UniProt IDA0KJL8
Gene NamephnW; Locus:AHA_1938
Protein Name2-aminoethylphosphonate--pyruvate transaminase
Region Expressed1-380
Expression Tag6xHis
Purity>90%
AA SequenceMTHIPAAPAAVDYLLLTPGPLSTTATVRAAMLQDSCTWDADYNQGVVEPIRRELVRLAAG PEYQSDYSAVLLQGSGSYVVESVLGSAIGVDECLLIINNGAYGARMGEMARCLGLRHHEL DCGETTRPEAAAIEAMLVRHPEITHLAMVHCETTTGMLNPLEEVAALCQRRGIRLIVDAM SSFGGIPIDMGRLGIEFLISSANKCIQGVPGFGFVIARRAALAACAGCARSVSLDLHAQW QTMEQQGGKWRFTSPTHTVLAFAQALRELDEEGGIAARHRRYRDNQRTLVDGMAALGFAP LLPEQWQSPIITAFYSPAHPDYRFADFYQRLKAQGFVIYPGKVSQADCFRIGNIGDVTPA RVRCLLVAMASACYWQGDAR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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